Coagulation analysis Instruction Sheet
INS-INP-007- Coagulation analysis Instruction sheet -V2.0	Version#: 2.0		Issue date: 2025		Language : English-Arabic
Subject form: Coagulation analysis Instruction sheet	Subject file:  INS-INP-007- Coagulation analysis Instruction sheet -V2.0		Pages # : 3				Related SOPs&Documents:
Prepared by: Ziyad Alkhdour		Verified by: Sawsan Khawaja		Qualified by: Duaa Mushalah		Approved by: Ziyad Alkhdour

 Dear Participant:

• Enclosed is your Clinical Coagulation specimen number (xxx) tested negative for hepatitis and HIV.

       Specimen Type

     Lyophilized citrated Plasma

       Specimen Storage

     Keep the specimen lyophilized until use and store it at 2-8 °C.

Reconstituting Lyophilized EQAS Material

The process of reconstitution involves adding a specified volume of distilled water to lyophilized QC material. The water should completely dissolve the lyophilized contents, giving a liquid solution, which is ready for analysis.

Reconstitution is a straightforward process, but requires a high level of precision. Small errors can have serious implications to the reconstituted material:

• If too much water is pipetted during reconstitution, the material will be heavily diluted and results will be lower than expected
• If too little water is pipetted during reconstitution, the material will not be sufficiently diluted, and results will be higher than expected
• If the correct volume of water is pipetted, but a small amount of water gets stuck in the pipette tip due to poor pipetting technique, results will be higher than expected

If a lyophilized control has been reconstituted incorrectly the contents of the vial will be wasted. It is therefore vitally important that controls are reconstituted with care.

Materials and Methods Required

The list of requirements for an accurate and consistent reconstitution technique is not extensive, but each requirement is vital. Labs should have:

• Calibrated volumetric pipettes
• Sterile, appropriately sized pipette tips
• Distilled water
• Technician with good pipetting technique
• Lyophilized EQAS stored at refrigerator (2-8 °C)

How to Reconstitute Lyophilised EQAS Material

Each different lyophilised control may require slightly different preparation, always refer to the instructions for use before reconstituting control material.  

1. Place the vial of lyophilized EQAS on a flat surface, carefully remove the lid and the rubber stopper making sure not to spill any material
2. Using a calibrated pipette and sterilized pipette tip, add exactly 1ml of distilled water directly into the EQAS vial, ensuring no water is left in the pipette tip, or on the rim/side of the vial

• Place the rubber stopper and lid firmly back onto the EQAS vial, and make sure that the specimen is completely dissolved then incubate at 37c at least for thirty minutes(30min) and swirl it gently every five minutes (5 min). Do not shake.
• The specimen is stable for two-hour(2hrs) at room temperature once dissolved.
• Complete the assay for analytes indicated on the accompanying Results Return Sheet.
• The tests are: PT, APPT.& INR.

1. Use the methods and equipment reported in the methodology Registration sheet.
2. On the Results Return Sheet record the date the sample was received and the date the sample was analyzed.
3. Write your results clearly in the appropriate result space ensuring they are in the units indicated.

Return your results to EQAS office:

* Through the web site of EQAS (www.eqas.alquds.edu)

* By fax: 02/2411149

* By E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

* By telephone: 02/2414076 or 0598583807

* By WhatsApp 970598583807

Method, reagent and instrument are entered into the fields. You will find the analysis key for methods and the key for reagents in this commentary. The entries in the indicated fields are important for the correct classification of results for a defined group for assessment. If other methods, reagents, or instruments have been used, which are not listed in the key, please indicate the reagents used. The fields for results are for entering your analytical results

For consultation and/or questions please contact      

Mr. Ziyad Al khdour On Sunday- Thursday. at 9:00- 15:00

Additional Considerations

It is important to remember that there may be slightly different reconstitution requirements for different EQAS material. For this reason, it is vital that the instructions provided on the Website are closely followed.

If you have any further questions regarding lyophilised controls or would like to contact us, please do so by emailing us at This email address is being protected from spambots. You need JavaScript enabled to view it. or you can ask specifically

 Mr. Ziyad Al khdour On Sunday- Thursday. at 9:00- 15:00

Written by Ziyad Khdour